Difference between revisions of "Mutagenesis"

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To mutate a residue follow these easy steps:
 
To mutate a residue follow these easy steps:
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#Load a PDB file
+
<OL>
−
#Under the '''Wizard''' menu select '''Mutagenesis'''
+
<LI>Load a PDB file<BR><BR>
−
#In the PyMol viewer window select a residue
+
 
−
#Select '''No Mutation''' and select resultant residue
+
[[Image:Mutag1.png|180px|none]]
−
#Select Apply
+
<LI>Under the '''Wizard''' menu select '''Mutagenesis'''<BR><BR>
−
#Select Done
+
 
−
[[Image:Mutag1.png|thumb|1. Wizard -> Mutagenesis]]
+
[[Image:Mutag2.png|180px|none]]
−
[[Image:Mutag2.png|thumb|2. Select the Residue]]
+
<LI>In the PyMol viewer window select a residue<BR><BR>
−
[[Image:Mutag3.png|thumb|3. No Mutation; choose residue]]
+
 
−
[[Image:Mutag4.png|thumb|4. Apply; Done]]
+
[[Image:Mutag3.png|180px|none]]
 +
<LI>Select '''No Mutation''' and select resultant residue<BR><BR>
 +
 
 +
[[Image:Mutag4.png|180px|none]]
 +
<LI>Select Apply
 +
<LI>Select Done
 +
</OL>

Revision as of 16:40, 11 May 2006

Mutagenesis

PyMol has a Mutagenesis Wizard to make mutagenesis very easy for the end user.

To mutate a residue follow these easy steps:

  1. Load a PDB file

    Mutag1.png
  2. Under the Wizard menu select Mutagenesis

    Mutag2.png
  3. In the PyMol viewer window select a residue

    Mutag3.png
  4. Select No Mutation and select resultant residue

    Mutag4.png
  5. Select Apply
  6. Select Done
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