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Biochemistry student intro
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== Background == This tutorial is designed to give you a basic working knowledge of making pretty and informative pictures of protein structures using PyMOL. <br> This tutorial does not cover all the functions of PyMol, but tries to focus on the most important ones. <br> A great resource for more advanced use is this wiki and the large number of good tutorials found online, which can be accessed via google.<br> When you open PyMOL you will see two windows opening.<br> The upper window entitled '''PyMOL Tcl/Tk GUI''' controls the general settings and functionalities of the program while the lower one entitled '''PyMOL viewer''', contains the settings that are related to the current display of the molecule. <br> Each of these two windows contain a command line, where we can enter commands into the program. <br> One can, however, get really far without ever having to worry about writing any commands. First, we will need to load a structure file into the program. Protein structures are deposited at [[www.pdb.org]]<br> There are several ways to open this file:<br> Either we can download the file from PDB and save it to our computer.<br> We can then open the PDB file (if you have it on the harddisk) using the menu '''File > Open'''.<br> or we can write: '''fetch''' followed by the PDB id in either of the two command lines. Ex: '''fetch 1LEV''' <br> The Viewer window should now contain the PDB file displayed with lines. In the right hand side of the viewer window there is a selection menu that currently contains two lines:<br> '''all''' and the four character name of the PDB file, ex. '''1lev'''.<br> Later on we will make new selections that will appear in this menu.<br> Each of the two lines have 5 buttons labeled '''A''' (Actions), '''S''' (Show), '''H''' (Hide), '''L''' (Label), '''C''' (Color).<br> Press these buttons to see the options available in each menu. '''Hide the line representation'''. Press '''H > everything''' in the '''all''' line. This will remove the line representation of the molecule.<br> '''Visualize the molecule in the cartoon representation''' by selecting '''S > cartoon'''.<br> Try also to show the molecule as ribbon, sticks or surface to get a feeling for the different representations.<br> The cartoon option gives us the best overview of the overall structure, so hide the other representations and show the protein only in cartoon mode.<br> Color everything white selecting '''C > grey > white'''. Ok, now we are going to try to move the molecule around. <br>Try to move the mouse around while pressing the left mouse button. This will rotate the molecule.<br> Try to move it around holding the middle button (moves the view) or the right button (zooms in and out).<br> If you have a one or two button mouse you can change the mode in the '''Mouse''' menu in the Tcl/Tk GUI window. <br> The box in the lower right corner will show how to move and rotate the molecule in this case.
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